[Preparation of floxuridine loaded polycation and its antitumor activity]

Zhejiang Da Xue Xue Bao Yi Xue Ban. 2009 Jan;38(1):53-8. doi: 10.3785/j.issn.1008-9292.2009.01.008.
[Article in Chinese]

Abstract

Objective: To develop a new prodrug of 5-fluorouracil-polyethylenimine-beta-cyclodextrin-floxuridine (PEI-beta-CyD-Fd) and to test its antitumor activity.

Methods: Floxuridine was conjugated to polyethylenimine-beta-cyclodextrin to form prodrug PEI-beta-CyD-Fd. The structure of synthesized PEI-beta-CyD-Fd was confirmed by (1)H-NMR, FT-IR and UV. MTT assay and cell wound healing assay were performed on human hepatic carcinoma cell line HepG2.

Result: The drug loading was 2 %. The MTT assay and cell wound healing assay indicated that PEI-beta-CyD-Fd significantly inhibited proliferation and migration of HepG2 cells.

Conclusion: The synthesized prodrug PEI-CyD-Fd has a significant antitumor activity on HepG2 cells.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antimetabolites, Antineoplastic / chemical synthesis*
  • Antimetabolites, Antineoplastic / pharmacology
  • Cell Line, Tumor
  • Cell Movement / drug effects
  • Cell Proliferation / drug effects
  • Floxuridine / pharmacology*
  • Fluorouracil / pharmacology*
  • Humans
  • Liver Neoplasms / pathology
  • Polyethyleneimine / pharmacology*
  • Prodrugs / chemical synthesis
  • Prodrugs / pharmacology
  • beta-Cyclodextrins / pharmacology*

Substances

  • Antimetabolites, Antineoplastic
  • Prodrugs
  • beta-Cyclodextrins
  • Floxuridine
  • Polyethyleneimine
  • Fluorouracil