A plethora of papers have been published that address the affects of chemicals on wildlife vertebrates. Collectively, they support a connection between environmental pollution and effects on wildlife vertebrate populations; however, causal relationships between exposure, and reproduction or population structure effects have been established for only a few species. In a vast number of fish species, particularly in teleosts, it is accepted that EDCs affect the endocrine system of individuals and may alter sexual development and fertility. However, only few studies have demonstrated population-level consequences as a result of exposure to EDCs. The same applies to fish populations exposed to contaminants or contaminant mixtures with non-endocrine modes of action; few studies link EDCs directly to population affects. Amphibian populations are declining in many parts of the world. Although environmental chemicals have been shown to affect reproduction and development in single organism tests, the degree to which chemicals contribute to the decline of amphibians, either alone, or in concert with other factors (habitat loss, climate change, introduction of neozoa, UV-B irradiation, and direct exploitation) is still uncertain. Because reptilian endocrinology is so variable among species, EDC effects reported for individual species cannot easily be extrapolated to others. Nevertheless, for some species and locations (e.g., the Lake Popka alligators), there is considerable evidence that population declines are caused or triggered by chemical pollution. In birds, there is ample evidence for EDC effects on the reproductive system. In some bird species, effects can be linked to population declines (e.g., based on egg-shell thinning induced by DDT/DDE). In contrast, other bird species were shown to be rather insensitive to endocrine disruption. Oil spills, which also may exert endocrine effects, are usually regarded to cause only transient bird population effects, although long-term data are largely missing. Mammal population declines have been correlated with organochlorine pollution. Moreover, numerous studies have attributed reproductive and non-reproductive dysfunctions in mammals to EDC exposure. However, in the majority of cases, it is uncertain if effects at the population level can be attributed to chemical-induced reproductive effects. Evidence shows that selected species from all vertebrate classes were negatively affected by certain anthropogenic chemicals. Affects on some species are well characterized at the organismal level. However, the proof of a direct link between chemical exposure and population decline was not given for the vast majority of studied species. This review clearly shows the gaps in knowledge that must be filled for the topic area addressed. We, herewith, make a plea for long-term studies to monitor effects of various environmental chemicals on wildlife vertebrate populations. Such studies may be augmented or combined with mechanistically-oriented histological, cytological and biochemical parallel investigations, to fill knowledge gaps.