High-speed counter-current chromatographic isolation of ricinine, an insecticide from Ricinus communis

Cristiane de Melo Cazal; Jaqueline Raquel Batalhão; Vanessa de Cássia Domingues; Odair Corrêa Bueno; Edson Rodrigues Filho; Moacir R Forim; Maria Fátima G Fernandes da Silva; Paulo Cezar Vieira; João Batista Fernandes

doi:10.1016/j.chroma.2009.02.008

High-speed counter-current chromatographic isolation of ricinine, an insecticide from Ricinus communis

J Chromatogr A. 2009 May 8;1216(19):4290-4. doi: 10.1016/j.chroma.2009.02.008. Epub 2009 Feb 10.

Authors

Cristiane de Melo Cazal¹, Jaqueline Raquel Batalhão, Vanessa de Cássia Domingues, Odair Corrêa Bueno, Edson Rodrigues Filho, Moacir R Forim, Maria Fátima G Fernandes da Silva, Paulo Cezar Vieira, João Batista Fernandes

Affiliation

¹ Universidade Federal de São Carlos, Departamento de Química, SP, Brazil.

PMID: 19249053
DOI: 10.1016/j.chroma.2009.02.008

Abstract

The alkaloid ricinine, an insecticide for leaf-cutting ant (Atta sexdens rubropilosa), was obtained from Ricinus communis. A two-phase solvent system composed of CH(2)Cl(2)/EtOH/H(2)O (93:35:72, v/v/v) was used for high-speed counter-current chromatographic (HSCCC) isolation of ricinine in high yield and with over 96% purity, as determined by liquid and gas chromatography-mass spectrometry (LC-MS and GC-MS). Identification of ricinine was performed by comparison of (1)H NMR, (13)C NMR and LC-MS/MS data.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alkaloids / isolation & purification*
Animals
Ants
Chromatography, Liquid
Countercurrent Distribution / methods*
Insecticides / isolation & purification*
Mass Spectrometry
Nuclear Magnetic Resonance, Biomolecular
Plant Extracts / chemistry
Plant Leaves / chemistry
Pyridones / isolation & purification*
Ricinus / chemistry*
Solvents / chemistry

Substances

Alkaloids
Insecticides
Plant Extracts
Pyridones
Solvents
ricinine