Abstract
Pen b 26 is one of the allergens produced by Penicillium brevicompactum which is one of the most prevalent in door airborne fungi and a major source of respiratory problems, including asthma. Pen b 26 wa scloned and expressed as an N-terminal as well as a C-terminal His6 tagged fusion protein in Escherichia coli. This allergen was purified by immobilized Ni2+-affinity chromatography. The purified Pen b 26 was characterized by immunological, biochemical and biophysical methods. C-His6 tagged Pen b 26 produced several fold greater yield than N-His6 tagged Pen b 26. The affinity of C-His6 tagged Pen b 26 for the specific antibody was also 2.75 times higher than N-His6 tagged Pen b 26
MeSH terms
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Allergens / biosynthesis*
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Allergens / chemistry*
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Allergens / genetics
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Allergens / immunology
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Allergens / isolation & purification
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Asthma / immunology
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Asthma / microbiology
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Escherichia coli / genetics
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Fungal Proteins / biosynthesis*
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Fungal Proteins / chemistry*
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Fungal Proteins / genetics
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Fungal Proteins / immunology
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Fungal Proteins / isolation & purification
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Gene Expression*
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Penicillium* / chemistry
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Penicillium* / genetics
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Penicillium* / immunology
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Recombinant Proteins / biosynthesis*
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Recombinant Proteins / chemistry*
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Recombinant Proteins / isolation & purification
Substances
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Allergens
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Fungal Proteins
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Recombinant Proteins