Microcrystalline chitosan (MCCh) characterise by many valuable features, including biocompatibility, antibacterial, fungicidal and high adhesive properties, high water retention value (WRV) factor, potent sorption, high bioactivity, biodegradability, ability to create membranes directly out of water suspensions. This biopolymer due to its biodegradability and safety in application no cytotoxic or immune interaction was assessed as a very effective haemostatic factor. MCCh with such properties form useful initial material for biological bandages; it can be used in surgery as growth factor carrier. Biodegradable mambranes are applied in surgery for guided tissue regeneration, what contributed to make the manufacturing technology of modified membranes out of microcrystalline chitosan. Peformed research was to show how certain angiogenic factors are released--platelet derived angiogenic factor (PDAF-AB) and transforming factor beta (TGF-beta) from doubled-layered alginate-chitosane membrane which was already used in dental surgery. Assessment of angiogenic factors release (PDGF-AB and TGF-beta) from polymer base may become helpful in choosing the right carrier for growth factors.