Abstract
Electron capture dissociation (ECD) allows fragmentation of the phosphopeptide backbone while keeping the labile phospho-amino acid intact. This feature of ECD fragmentation, coupled with the acquisition of mass spectra at high mass accuracy, makes ECD well-suited to phosphorylation mapping. The following methods are designed to focus ECD events on phosphopeptides within a complex peptide sample, either by using phosphoric acid neutral loss peaks as a trigger or by targeted analysis of predetermined precursor masses.
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Amino Acid Sequence
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Animals
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Catalytic Domain
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Chromatography, Liquid / methods*
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Electrons
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Humans
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Online Systems*
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Phosphopeptides / chemistry
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Phosphopeptides / isolation & purification
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Phosphoproteins / analysis*
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Phosphoproteins / chemistry*
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Phosphoproteins / isolation & purification
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Phosphoproteins / metabolism
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Phosphorylation
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Protein Kinases / metabolism
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Proteomics / methods
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Sequence Analysis, Protein / methods*
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Software
Substances
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Phosphopeptides
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Phosphoproteins
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Protein Kinases