Restriction fragment length polymorphism was used for evaluation of genetic heterogeneity of Coxiella burnettii strains. A 594-b. p. fragment of groEL gene was cleaved with MN1I, HpaII, and Hin6I endonucleases. Genetic homogeneity of Coxiella burnettii strains from the Russian collection and their relation to the group of European and North American strains were detected.