Abstract
The effect of lectins on the formation of osteoclasts in RAW 264.7 mouse macrophage cells was examined. Concanavalin A (Con A) induced the formation of multinucleated giant cells (MGC) whereas pokeweed mitogen and phytohemagglutinin did not do it. Con A-induced MGC were positive for tartrate- resistant acid phosphatase (TRAP) activity, a histochemical marker of osteoclasts. Murine splenic macrophages differentiated into TRAP-positive and multinucleated cells in response to Con A whereas peritoneal macrophages did not. The culture supernatant from Con A-stimulated RAW 264.7 cells did not cause the MGC formation. The relationship between Con A-induced GMC formation and osteoclastgenesis is discussed.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Acid Phosphatase / metabolism
-
Animals
-
Cell Differentiation / drug effects*
-
Cell Line
-
Concanavalin A / pharmacology*
-
Culture Media, Conditioned / pharmacology
-
Giant Cells / cytology
-
Giant Cells / metabolism
-
Isoenzymes / metabolism
-
Macrophages / cytology*
-
Macrophages / drug effects
-
Macrophages, Peritoneal / cytology
-
Macrophages, Peritoneal / drug effects
-
Mice
-
Mitogen-Activated Protein Kinases / antagonists & inhibitors
-
Osteoclasts / cytology*
-
Osteoclasts / metabolism
-
Phytohemagglutinins / pharmacology
-
Pokeweed Mitogens / pharmacology
-
Protein Kinase Inhibitors / pharmacology
-
Signal Transduction / drug effects
-
Tartrate-Resistant Acid Phosphatase
Substances
-
Culture Media, Conditioned
-
Isoenzymes
-
Phytohemagglutinins
-
Pokeweed Mitogens
-
Protein Kinase Inhibitors
-
Concanavalin A
-
Mitogen-Activated Protein Kinases
-
Acid Phosphatase
-
Acp5 protein, mouse
-
Tartrate-Resistant Acid Phosphatase