Concanavalin A induces formation of osteoclast-like cells in RAW 264.7 mouse macrophage cells

Immunopharmacol Immunotoxicol. 2009;31(1):103-7. doi: 10.1080/08923970802438417.

Abstract

The effect of lectins on the formation of osteoclasts in RAW 264.7 mouse macrophage cells was examined. Concanavalin A (Con A) induced the formation of multinucleated giant cells (MGC) whereas pokeweed mitogen and phytohemagglutinin did not do it. Con A-induced MGC were positive for tartrate- resistant acid phosphatase (TRAP) activity, a histochemical marker of osteoclasts. Murine splenic macrophages differentiated into TRAP-positive and multinucleated cells in response to Con A whereas peritoneal macrophages did not. The culture supernatant from Con A-stimulated RAW 264.7 cells did not cause the MGC formation. The relationship between Con A-induced GMC formation and osteoclastgenesis is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acid Phosphatase / metabolism
  • Animals
  • Cell Differentiation / drug effects*
  • Cell Line
  • Concanavalin A / pharmacology*
  • Culture Media, Conditioned / pharmacology
  • Giant Cells / cytology
  • Giant Cells / metabolism
  • Isoenzymes / metabolism
  • Macrophages / cytology*
  • Macrophages / drug effects
  • Macrophages, Peritoneal / cytology
  • Macrophages, Peritoneal / drug effects
  • Mice
  • Mitogen-Activated Protein Kinases / antagonists & inhibitors
  • Osteoclasts / cytology*
  • Osteoclasts / metabolism
  • Phytohemagglutinins / pharmacology
  • Pokeweed Mitogens / pharmacology
  • Protein Kinase Inhibitors / pharmacology
  • Signal Transduction / drug effects
  • Tartrate-Resistant Acid Phosphatase

Substances

  • Culture Media, Conditioned
  • Isoenzymes
  • Phytohemagglutinins
  • Pokeweed Mitogens
  • Protein Kinase Inhibitors
  • Concanavalin A
  • Mitogen-Activated Protein Kinases
  • Acid Phosphatase
  • Acp5 protein, mouse
  • Tartrate-Resistant Acid Phosphatase