Oxolinic acid (OA) and flumequine (FLU) are two of the quinolone antibiotics (QAs) that are widely used in aquaculture. The purpose of this study was to understand the fates of OA and FLU in waters and sediment slurries from aquaculture ponds in a laboratory experiment. Waters and sediments were sampled from an eel (Anguilla japonica) pond and a shrimp (Penaeus vannamei) pond. The effects of light, microbial activities, and temperature on the degradation of these two QAs were elucidated. Results indicated that light plays a major role in the degradation of OA and FLU in waters and sediment slurries. Under illuminated and non-sterile conditions, the half-lives (t(1/2)) of OA were 2.3-4.8 and 9.5-15.0 days in the waters and sediment slurries, respectively. For FLU, under the same conditions, t(1/2) values were 1.9-2.3 and 3.6-6.4 days, respectively. Photodegradation of OA and FLU was much faster in water than in sediment slurry. In both environments, degradation became very slow or would plateau after only minimal change in the dark. Besides the effect of light, biodegradation had very minor effects on the degradation of the two QAs in the sediment slurries. The only independent biodegradation was found when OA was placed in shrimp pond sediment slurry, but at a much lower rate (t(1/2) of 98.7 days) than in light. Biodegradation of FLU was also found in the eel pond sediment slurry but only through an additional connection with light. Also, re-addition enhanced the degradation of OA in shrimp pond sediment slurry, but slowed the degradation of FLU in the eel pond sediment slurry in the dark. The temperature experiment in this study showed no significant effects on degradation of the two QAs in either pond waters or sediment slurries.