GLyphosate (N-phosphonomethyl-glycine) is a systemic, broad spectrum herbicide effective against most plant species, including annual and perennial species and is one of the world's most widely used herbicide. To glyphosate applied treatments, a part of active agent comes in contact with soil surface, adsorbing to soil compounds, while another part remains in soil solution. The adsorbing to soil compounds represents a feat importance conditioning the herbicide presence in soil solution and so, his availability to degradation and dispersion in the environment. In this paper work, the extractable glyphosate residues from soil solution have been determined through HPLC-FLD. Substrates used were Black Chernozem, Typical Gleysoil, Slight Vertisol, with moderate carbonatation. The experimental results indicated that the extractable glyphosate residue fractions from soil diminish (<20%), depending of soil parameters and decrease in this order: Gleysoil, Black Chernozem, Slight Vertisol.