Structural organization of a complex family of palindromic repeats in Enterococci

FEMS Microbiol Lett. 2009 Mar;292(1):7-12. doi: 10.1111/j.1574-6968.2008.01461.x.

Abstract

Enterococcus faecalis/faecium repeats (EFARs) are miniature insertion sequences spread in the genome of Enterococcus faecalis and Enterococcus faecium. Unit-length repeats measure 165-170 bp and contain two modules (B and T) capable of folding independently into stem-loop sequences, connected by a short, unstructured module J. The E. faecalis elements feature only one type of B, J and T modules. In contrast, the E. faecium elements result from the assembly of different types of B, J and T modules, and may vary in length because they carry multiple B modules. Most EFARs are located close (0-20 bp) to ORF stop codons, and are thus cotranscribed with upstream flanking genes. In both E. faecalis and E. faecium cells, EFAR transcripts accumulate in a strand-dependent fashion. Data suggest that T modules function as bidirectional transcriptional terminators, which provide a 3'-end to gene transcripts spanning B modules, while blocking antisense transcripts coming in from the opposite direction.

MeSH terms

  • Base Sequence
  • DNA, Bacterial / genetics*
  • Enterococcus faecalis / genetics*
  • Enterococcus faecium / genetics*
  • Inverted Repeat Sequences*
  • Molecular Sequence Data
  • Sequence Alignment

Substances

  • DNA, Bacterial