Abstract
We have characterized the gene emperor's thumb (et) and showed that it is required for the regulation of apoptosis in Drosophila. Loss-of-function mutations in et result in apoptosis associated with a decrease in the concentration of DIAP1. Overexpression of one form of et inhibits apoptosis, consistent with et having an anti-apoptotic function; however, overexpression of a second form of et induces apoptosis, indicating that the two forms of et may have competing functions. et encodes a protein deubiquitinase, suggesting it regulates apoptosis by controlling the stability of apoptotic regulatory proteins.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Apoptosis / physiology*
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Apoptosis Regulatory Proteins / physiology*
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Drosophila / genetics
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Drosophila / metabolism*
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Drosophila Proteins / physiology*
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Endopeptidases / physiology*
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Genes, Insect*
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Models, Biological
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Molecular Sequence Data
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Mutation
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Phylogeny
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Protein Structure, Tertiary
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Sequence Homology, Amino Acid
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Ubiquitin-Protein Ligases / metabolism
Substances
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Apoptosis Regulatory Proteins
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Drosophila Proteins
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Ubiquitin-Protein Ligases
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Endopeptidases
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ubiquitin specific protease 36, Drosophila