Hyperbaric oxygen treatment induces platelet aggregation and protein release, without altering expression of activation molecules

Frances L Shaw; Paul G Winyard; Gary R Smerdon; Philip J Bryson; A John Moody; Paul Eggleton

doi:10.1016/j.clinbiochem.2008.12.024

Hyperbaric oxygen treatment induces platelet aggregation and protein release, without altering expression of activation molecules

Clin Biochem. 2009 Apr;42(6):467-76. doi: 10.1016/j.clinbiochem.2008.12.024. Epub 2009 Jan 8.

Authors

Frances L Shaw¹, Paul G Winyard, Gary R Smerdon, Philip J Bryson, A John Moody, Paul Eggleton

Affiliation

¹ Institute of Biomedical and Clinical Science, Peninsula College of Medicine and Dentistry, University of Exeter, Exeter, UK. Frances.Shaw@manchester.ac.uk

PMID: 19210959
DOI: 10.1016/j.clinbiochem.2008.12.024

Abstract

Objectives: To investigate the effect of hyperbaric oxygen (HBO) on platelet physiology.

Design and methods: Human platelets were exposed to HBO (97.7% O(2), balance CO(2) at 2.2 ata) or control (CON; 5% CO(2), balance air at 1 ata) for 90 min, and analyzed for aggregation, protein release, ()NO production, and activation.

Results: HBO induced 29.8+/-3.0% of platelets to aggregate compared with CON (5.5+/-0.9%). Proteins observed to be released in greater abundance from HBO- compared with CON-treated platelets included 14-3-3 zeta and alpha-2-macroglobulin. Release of ()NO by platelets was unaffected following exposure to HBO, as was platelet activation as measured by surface expression of PECAM-1, CD62P and the activated form of alpha(IIB)beta(IIIa).

Conclusions: Exposure to HBO induces both platelet aggregation and protein release. Further study will better define the precise mechanisms and effects of HBO on platelet activation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

14-3-3 Proteins / metabolism
Blood Platelets / chemistry
Blood Platelets / physiology*
Blood Proteins / metabolism*
Humans
Hyperbaric Oxygenation*
Membrane Glycoproteins / biosynthesis*
Nitrates / analysis
Nitric Oxide / biosynthesis
Nitrites / analysis
P-Selectin / biosynthesis
Platelet Activation
Platelet Aggregation*
Platelet Endothelial Cell Adhesion Molecule-1 / biosynthesis
Platelet Glycoprotein GPIIb-IIIa Complex / biosynthesis
Platelet-Rich Plasma / chemistry
alpha-Macroglobulins / metabolism

Substances

14-3-3 Proteins
Blood Proteins
Membrane Glycoproteins
Nitrates
Nitrites
P-Selectin
Platelet Endothelial Cell Adhesion Molecule-1
Platelet Glycoprotein GPIIb-IIIa Complex
alpha-Macroglobulins
Nitric Oxide