Abstract
Sequential multiplex PCR was evaluated for detection of multiple Streptococcus pneumoniae serotypes directly from nasopharyngeal secretions. A total of 279 nasopharyngeal swab samples were tested blindly. When limited to the 29 serotypes identifiable by the molecular method, the mean number of serotypes identified by the conventional latex/Quellung method was 0.85, which was significantly lower than that by the molecular method (P <0.0001). The multiplex PCR method identified significantly more serotypes than the latex/Quellung method if limited to the 29 serotypes (P=0.001 and P=0.014, respectively).
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Binomial Distribution
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Carrier State / microbiology*
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Cross-Sectional Studies
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DNA, Bacterial / isolation & purification
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Gambia
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Humans
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Latex Fixation Tests
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Nasopharynx / microbiology*
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Pneumococcal Infections / microbiology*
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Pneumococcal Vaccines / standards
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Polymerase Chain Reaction / methods*
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Probability
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Sensitivity and Specificity
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Serotyping
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Streptococcus pneumoniae / classification
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Streptococcus pneumoniae / genetics
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Streptococcus pneumoniae / isolation & purification*
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Vaccines, Conjugate / standards
Substances
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DNA, Bacterial
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Pneumococcal Vaccines
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Vaccines, Conjugate