Crystallographic analysis of the neurophysin-oxytocin complex. A preliminary report

J P Rose; E Breslow; H B Huang; B C Wang

doi:10.1016/0022-2836(91)80201-5

Crystallographic analysis of the neurophysin-oxytocin complex. A preliminary report

J Mol Biol. 1991 Sep 5;221(1):43-5. doi: 10.1016/0022-2836(91)80201-5.

Authors

J P Rose¹, E Breslow, H B Huang, B C Wang

Affiliation

¹ Department of Crystallography, University of Pittsburgh, PA 15260.

PMID: 1920416
DOI: 10.1016/0022-2836(91)80201-5

Abstract

Single crystals of a bovine neurophysin II-oxytocin complex have been obtained using (NH4)2SO4 as the precipitating agent. The crystals diffract to at least 2.7 A resolution, belong to Laue group 4/mmm and exhibit systematic absences consistent with either space group P4(1)2(1)2 or P4(3)2(1)2. The cell dimensions are a = b = 69.07 A and c = 113.26 A. The crystals contain one neurophysin-oxytocin dimer per asymmetric unit. Based on a Vm of 2.9 A3/Da, the solvent content is calculated to be 58%. Chromatographic analysis of the dissolved crystals suggests the presence of three oxytocin molecules per neurophysin dimer.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Animals
Cattle
Neurophysins / chemistry*
X-Ray Diffraction

Substances

Neurophysins

Abstract

Publication types

MeSH terms

Substances

Grants and funding