Abstract
A fluorescent Zn(2+) sensor based on the 2-(2'-pyridinyl)benzoimidazole (2-PBI) fluorophore has been devised by incorporation with a Zn(2+) ionophore, bis(pyridin-2-ylmethyl)amine. The sensor (PBITA) demonstrates a Zn(2+)-specific emission shift and enhancement with a 1:1 binding ratio. Due to the Zn(2+)-induced coplanation of 2-PBI via reversion/rotation, PBITA is shown to behave as a ratiometric sensor. The intracellular Zn(2+) imaging ability of the sensor has been tested in HeLa cells using a confocal microscope.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Benzimidazoles / chemical synthesis*
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Benzimidazoles / chemistry
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Benzimidazoles / pharmacology*
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Fluorescent Dyes / chemical synthesis*
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Fluorescent Dyes / chemistry
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Fluorescent Dyes / pharmacology
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HeLa Cells
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Humans
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Microscopy, Confocal
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Molecular Conformation
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Molecular Structure
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Pyridines / chemical synthesis*
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Pyridines / chemistry
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Pyridines / pharmacology*
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Zinc / chemistry*
Substances
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2-(2'-pyridinyl)benzoimidazole
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Benzimidazoles
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Fluorescent Dyes
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Pyridines
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bis(pyridin-2-ylmethyl)amine
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Zinc