MeCN, acetonitrile; ECL, enhanced chemiluminescence; EDT, 1,2-ethanedithiole; HEPC12-A, rabbit anti-human hepcidin IgG, affinity purified; HEPC13-A, rabbit anti-mouse/human hepcidin IgG, affinity purified; HEPC61-P, human hepcidin-25 control/blocking synthetic peptide; HRP, horseradish peroxidase; IL-6, interleukin-6; KLH, keyhole limpet hemocyanin; LEAP, liver-expressed antimicrobial peptide; NEM, N-ethylmaleimide; NMP, N-methyl-pirrolidone; PBS, phosphate buffered saline; PVDF, polyvinylidene difluoride; SELDI-TOF-MS, surface-enhanced laser desorption ionization-time-of-flight mass spectrometry; TMB, tetramethylbenzidin; TNF-alpha, tumor necrosis factor-alpha.