Abstract
Entamoeba histolytica and Entamoeba dispar are two microscopically indistinguishable amoebae living in the human colon. The former is a pathogen, whereas the latter is a nonpathogenic commensal. Using a model system of in vitro cocultures and PCR detection of the Entamoeba species, we found that the nonpathogenic species can rapidly outgrow the pathogen in xenic cultures.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Coculture Techniques
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Culture Media
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DNA, Protozoan / analysis
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DNA, Protozoan / isolation & purification
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Entamoeba / classification
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Entamoeba / genetics
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Entamoeba / growth & development*
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Entamoeba histolytica / classification
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Entamoeba histolytica / genetics
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Entamoeba histolytica / growth & development*
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Entamoeba histolytica / pathogenicity
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Entamoebiasis / diagnosis
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Entamoebiasis / parasitology
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Feces / parasitology
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Humans
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Polymerase Chain Reaction / methods
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Species Specificity
Substances
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Culture Media
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DNA, Protozoan