Abstract
An aqueous extract of Cornus kousa Burg. leaves (ACK) that contained high amount of polyphenols showed significant antioxidant activity against diphenylpicrylhydrazyl (DPPH) radicals and TNF-alpha-generated reactive oxygen species. ACK at concentrations of 10 and 50 microg/mL significantly inhibited TNF-alpha-induced adhesion of U937 pre-monocytic cells to HT-29 colon epithelial cells in a concentration-dependent manner. The reduced adhesion by ACK correlated with the suppressed expressions of monocyte chemoattractant protein (MCP)-1 and interleukin (IL)-8, the major inflammatory bowel disease (IBD)-associated chemokines. Moreover, ACK significantly suppressed TNF-alpha-induced translocation of redox-sensitive nuclear factor (NF)-kappaB as well as degradation of cytosolic I-kappaBalpha. The effective concentrations of ACK were much lower than that of 5-aminosalicylic acid (3.06 mg/mL), which is an active metabolite of sulfasalazine, a well-known drug used in the treatment of IBD. The results indicate that ACK may provide a potential benefit for the prevention and treatment of inflammatory diseases such as IBD.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Inflammatory Agents / isolation & purification
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Anti-Inflammatory Agents / pharmacology*
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Antioxidants / pharmacology
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Cell Adhesion / drug effects*
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Chemokine CCL2 / metabolism
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Chemokines / genetics
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Chemokines / metabolism*
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Colon / drug effects*
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Colon / immunology
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Colon / pathology
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Cornus* / chemistry
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Dose-Response Relationship, Drug
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Epithelial Cells / drug effects*
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Epithelial Cells / immunology
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Epithelial Cells / pathology
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Flavonoids / analysis
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HT29 Cells
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Humans
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I-kappa B Proteins / metabolism
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Interleukin-8 / metabolism
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Mesalamine / pharmacology
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Monocytes / drug effects*
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Monocytes / immunology
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NF-KappaB Inhibitor alpha
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NF-kappa B / metabolism
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Phenols / analysis
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Plant Extracts / pharmacology
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Plant Leaves
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Polyphenols
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RNA, Messenger / metabolism
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Tumor Necrosis Factor-alpha / metabolism*
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U937 Cells
Substances
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Anti-Inflammatory Agents
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Antioxidants
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CCL2 protein, human
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CXCL8 protein, human
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Chemokine CCL2
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Chemokines
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Flavonoids
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I-kappa B Proteins
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Interleukin-8
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NF-kappa B
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NFKBIA protein, human
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Phenols
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Plant Extracts
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Polyphenols
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RNA, Messenger
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Tumor Necrosis Factor-alpha
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NF-KappaB Inhibitor alpha
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Mesalamine