Abstract
Cell detachment is central to a broad range of physiopathological changes, but there are no quantitative methods to study this process. Here we report programmed subcellular release, a method for spatially and temporally controlled cellular detachment, and present quantitative results of the detachment dynamics of 3T3 fibroblasts at the subcellular level.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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3T3 Cells
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ADP Ribose Transferases / pharmacology
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Actin Cytoskeleton / drug effects
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Actin Cytoskeleton / physiology
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Actomyosin / physiology
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Algorithms
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Animals
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Azepines / pharmacology
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Botulinum Toxins / pharmacology
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Bridged Bicyclo Compounds, Heterocyclic / pharmacology
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Cell Adhesion / drug effects
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Cell Adhesion / physiology*
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Cell Movement / physiology
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Cell Shape / drug effects
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Cell Shape / physiology
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Cytological Techniques / methods*
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Electric Stimulation
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Electrodes
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Enzyme Inhibitors / pharmacology
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Fibroblasts / cytology
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Fibroblasts / drug effects
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Fibroblasts / physiology
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Focal Adhesions / drug effects
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Focal Adhesions / physiology
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Glass / chemistry
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Gold / chemistry
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Heterocyclic Compounds, 4 or More Rings / pharmacology
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Kinetics
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Mice
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Naphthalenes / pharmacology
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Oligopeptides / chemistry
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Stress Fibers / physiology
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Thiazolidines / pharmacology
Substances
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Azepines
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Bridged Bicyclo Compounds, Heterocyclic
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Enzyme Inhibitors
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Heterocyclic Compounds, 4 or More Rings
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Naphthalenes
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Oligopeptides
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Thiazolidines
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ML 7
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blebbistatin
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Gold
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arginyl-glycyl-aspartic acid
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Actomyosin
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ADP Ribose Transferases
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exoenzyme C3, Clostridium botulinum
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Botulinum Toxins
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latrunculin B