Objective: To study the influencing factors in preparation of brucine liposomes by ammonium sulfate transmembrane gradients.
Methods: The brucine liposomes were separated by Sephadex G-50, and the influence of various factors on the entrapment efficiencies were investigated.
Results: The entrapment efficiency was enhanced by increased ammonium sulfate concentration, ethanol volume and PC concentration.
Conclusion: Burcine liposomes prepared by ammonium sulfate transmembrance gradients can get a high entrapment efficiency, the main influencing factors were ammonium sulfate concentration, ethanol volume and PC concentration.