Mechanical property analysis of stored red blood cell using optical tweezers

Yanjie Li; Cheng Wen; Huimin Xie; Anpei Ye; Yajun Yin

doi:10.1016/j.colsurfb.2008.11.012

Mechanical property analysis of stored red blood cell using optical tweezers

Colloids Surf B Biointerfaces. 2009 May 1;70(2):169-73. doi: 10.1016/j.colsurfb.2008.11.012. Epub 2008 Nov 25.

Authors

Yanjie Li¹, Cheng Wen, Huimin Xie, Anpei Ye, Yajun Yin

Affiliation

¹ School of Aerospace, FML, Tsinghua University, Beijing, China.

PMID: 19168336
DOI: 10.1016/j.colsurfb.2008.11.012

Abstract

The deformation of human red blood cells subjected to direct stretching by optical tweezers was analyzed. The maximum force exerted by optical tweezers on the cell via a polystyrene microbead 5microm in diameter was 315pN. Digital image correlation (DIC) method was introduced to calculate the force and the deformation of the cell for the first time. Force-extension relation curves of the biconcave cell were quantitatively assessed when erythrocytes were stored in Alsever's Solution for 2 days, 5 days, 7 days and 14 days respectively. Experiment results demonstrated that the deformability of red blood cells was impaired with the stored time.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Blood Preservation / instrumentation
Blood Preservation / methods*
Calibration
Computer Simulation
Equipment Design
Erythrocyte Deformability
Erythrocyte Membrane / metabolism
Humans
Optical Tweezers*
Polystyrenes / chemistry
Stress, Mechanical
Time Factors

Substances

Polystyrenes