Abstract
CMV-seronegative subjects vaccinated intramuscularly or intradermally with a DNA vaccine encoding pp65, IE1, and gB were administered live-attenuated CMV (Towne) to characterize immune priming by the DNA vaccine. CMV-specific memory T-cells (detected by standard ELISPOT assay in only 20% of subjects) were detected by IFN-gamma cultured ELISPOT assay in 60% of subjects primed intramuscularly and correlated with immune responses after Towne. The median time to first pp65 T-cell and gB antibody response after Towne was 14 days for DNA-primed subjects vs. 28 days for controls administered Towne only (p=0.02 and 0.03, respectively). Furthermore, there was a trend toward more DNA-vaccinated subjects than controls developing a gB-specific IFN-gamma T-cell response after Towne administration (47% vs. 0%, p=0.06).
Publication types
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Randomized Controlled Trial
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Antibodies, Viral / biosynthesis
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Antigens, Viral
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Cytomegalovirus / immunology*
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Cytomegalovirus Vaccines / administration & dosage*
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Cytomegalovirus Vaccines / adverse effects
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Cytomegalovirus Vaccines / immunology
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Female
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Humans
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Immunologic Memory*
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In Vitro Techniques
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Injections, Intradermal
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Injections, Intramuscular
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Interferon-gamma / biosynthesis
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Male
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T-Lymphocytes / immunology
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Vaccines, Attenuated / administration & dosage
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Vaccines, Attenuated / adverse effects
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Vaccines, Attenuated / immunology
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Vaccines, DNA / administration & dosage
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Vaccines, DNA / adverse effects
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Vaccines, DNA / immunology
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Young Adult
Substances
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Antibodies, Viral
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Antigens, Viral
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Cytomegalovirus Vaccines
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Vaccines, Attenuated
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Vaccines, DNA
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Interferon-gamma