Production of monoclonal antibody to Clostridium difficile toxin A which neutralizes enterotoxicity but not haemagglutination activity

FEMS Microbiol Lett. 1991 Jul 1;65(3):311-5. doi: 10.1016/0378-1097(91)90233-z.

Abstract

Nine monoclonal antibodies (mAb) to Clostridium difficile toxin A were produced. The isotype of one mAb (37B5) was IgG2b, kappa, and that of the other eight mAbs was IgM, kappa. Immunoblot analysis after non-denatured PAGE showed that with the exception of one mAb (112G6) all mAbs gave a positive reaction with the 540 kDa band of toxin A. Immunoblot analysis showed that four mAbs (2E15, 3B4, 37B5 and 49C4) gave a positive reaction with the 240 kDa major band of toxin A. In neutralisation tests with these mAbs for enterotoxicity, mouse lethality, haemagglutination activity and cytotoxicity, 37B5 neutralised enterotoxicity in a rabbit ileal loop response test but did not neutralise any other biological activities. None of the other eight mAbs showed any neutralising activities at all.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / biosynthesis
  • Antibodies, Monoclonal / immunology*
  • Bacterial Toxins*
  • Blotting, Western
  • Clostridioides difficile / immunology*
  • Cross Reactions
  • Electrophoresis, Polyacrylamide Gel
  • Enterotoxins / antagonists & inhibitors
  • Enterotoxins / immunology*
  • Enterotoxins / isolation & purification
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Hemagglutination / immunology*
  • Ileum / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Neutralization Tests
  • Rabbits

Substances

  • Antibodies, Monoclonal
  • Bacterial Toxins
  • Enterotoxins
  • tcdA protein, Clostridium difficile