Immunochromatographic lateral flow strip tests

Methods Mol Biol. 2009:504:169-83. doi: 10.1007/978-1-60327-569-9_12.

Abstract

The immunochromatographic lateral flow strip test is a one-step test that facilitates low-cost, rapid identification of various analytes at the point of care. We have developed lateral flow strip tests for the specific qualitative or semiquantitative detection of antigens, antibodies, and haptens, such as drug residues. Here, we describe in detail the preparation of three examples of the strip tests for detection of (a) the infectious bursal disease virus; (b) Trichinella specific antibodies, and (c) Clenbuterol residues in urine samples.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / instrumentation*
  • Biosensing Techniques / methods
  • Chromatography / methods*
  • Equipment Design
  • Equipment Failure Analysis
  • Immunoassay / instrumentation*
  • Point-of-Care Systems*
  • Reagent Strips*
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • Reagent Strips