Receptor-mediated targeting of cathepsins in professional antigen presenting cells

Ulrik Hillaert; Martijn Verdoes; Bogdan I Florea; Athanasios Saragliadis; Kim L L Habets; Johan Kuiper; Serge Van Calenbergh; Ferry Ossendorp; Gijsbert A van der Marel; Christoph Driessen; Hermen S Overkleeft

doi:10.1002/anie.200805529

Receptor-mediated targeting of cathepsins in professional antigen presenting cells

Angew Chem Int Ed Engl. 2009;48(9):1629-32. doi: 10.1002/anie.200805529.

Authors

Ulrik Hillaert¹, Martijn Verdoes, Bogdan I Florea, Athanasios Saragliadis, Kim L L Habets, Johan Kuiper, Serge Van Calenbergh, Ferry Ossendorp, Gijsbert A van der Marel, Christoph Driessen, Hermen S Overkleeft

Affiliation

¹ Leiden Institute of Chemistry, Leiden University, P.O. Box 9052, 2300 RA Leiden, The Netherlands.

PMID: 19156780
DOI: 10.1002/anie.200805529

Abstract

Tag for professionals: A fluorescently tagged clustered mannoside DCG-04 analogue (see structure) is designed and synthesized using a modular approach. Uptake of the probe in professional antigen presenting cells and subsequent labeling of cathepsins proceeded in a mannose-receptor dependent manner.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigen-Presenting Cells / immunology*
Cathepsins / metabolism*
Cells, Cultured
Lectins, C-Type / metabolism
Leucine / analogs & derivatives
Leucine / chemical synthesis
Leucine / chemistry
Leucine / pharmacology
Mannose Receptor
Mannose-Binding Lectins / metabolism
Rats
Receptors, Cell Surface / metabolism

Substances

DCG 04
Lectins, C-Type
Mannose Receptor
Mannose-Binding Lectins
Receptors, Cell Surface
Cathepsins
Leucine