Abstract
Here we integrated multiphoton laser scanning microscopy and the registration of second harmonic generation images of collagen fibers to overcome difficulties in tracking stromal cell-matrix interactions for several days in live mice. We show that the matrix-modifying hormone relaxin increased tumor-associated fibroblast (TAF) interaction with collagen fibers by stimulating beta1-integrin activity, which is necessary for fiber remodeling by matrix metalloproteinases.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Line, Tumor
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Cell Movement / physiology
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Collagen / metabolism*
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Extracellular Matrix / metabolism
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Extracellular Matrix / pathology*
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Fibroblasts
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Humans
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Image Enhancement / methods
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Integrin beta Chains / metabolism
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Mice
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Mice, Transgenic
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Microscopy, Confocal / methods*
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Relaxin / pharmacology
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Soft Tissue Neoplasms / metabolism
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Soft Tissue Neoplasms / pathology*
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Stromal Cells / metabolism
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Stromal Cells / pathology
Substances
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Integrin beta Chains
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Relaxin
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Collagen