Factors responsible for plaque formation of A/duck/Siberia/272/1998 (H13N6) influenza virus on MDCK cells

Yoshimi Tsuda; Norikazu Isoda; Yoshihiro Sakoda; Hiroshi Kida

doi:10.1016/j.virusres.2008.12.005

Factors responsible for plaque formation of A/duck/Siberia/272/1998 (H13N6) influenza virus on MDCK cells

Virus Res. 2009 Mar;140(1-2):194-8. doi: 10.1016/j.virusres.2008.12.005. Epub 2009 Jan 13.

Authors

Yoshimi Tsuda¹, Norikazu Isoda, Yoshihiro Sakoda, Hiroshi Kida

Affiliation

¹ Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Nishi-9, Kita-18, Kita-ku, Sapporo 060-0818, Japan.

PMID: 19146891
DOI: 10.1016/j.virusres.2008.12.005

Abstract

Many influenza A viruses form plaques on Madin-Darby canine kidney (MDCK) cells in the presence of trypsin. A/duck/Siberia/272/1998 (H13N6) (Sib272), however, does not form plaque on MDCK cells. After three blind passages of the strain on MDCK cells, plaque-forming variant was obtained and designated as A/duck/Siberia/272PF/1998 (H13N6) (Sib272PF). Genetic and functional analyses of Sib272 and Sib272PF revealed that amino acid substitutions, F3L of the HA2 subunit and T379K of the PB1, were responsible for plaque formation of Sib272PF by enhancing fusion and polymerase activities, respectively.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Substitution
Animals
Cell Line
Dogs
Hemagglutinin Glycoproteins, Influenza Virus / genetics*
Humans
Influenza A virus / genetics*
Influenza A virus / growth & development
Orthomyxoviridae Infections / virology
RNA, Viral / genetics
RNA-Dependent RNA Polymerase / genetics*
Sequence Analysis, RNA
Viral Plaque Assay
Viral Proteins / genetics*
Virus Cultivation

Substances

Hemagglutinin Glycoproteins, Influenza Virus
PB2 protein, Influenzavirus A
RNA, Viral
Viral Proteins
RNA-Dependent RNA Polymerase