Sulfated modification of the polysaccharides obtained from defatted rice bran and their antitumor activities

Li Wang; Xiaoxuan Li; Zhengxing Chen

doi:10.1016/j.ijbiomac.2008.12.006

Sulfated modification of the polysaccharides obtained from defatted rice bran and their antitumor activities

Int J Biol Macromol. 2009 Mar 1;44(2):211-4. doi: 10.1016/j.ijbiomac.2008.12.006. Epub 2008 Dec 24.

Authors

Li Wang¹, Xiaoxuan Li, Zhengxing Chen

Affiliation

¹ State Key Laboratory of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, China.

PMID: 19135473
DOI: 10.1016/j.ijbiomac.2008.12.006

Abstract

Nine sulfated defatted rice bran polysaccharides (sRBPS), with various degrees of sulfation (DS) and carbohydrate content, were prepared by chlorosulfonic acid-pyridine (CSA-Pyr) method according to orthogonal test. Nine sulfated derivatives sRBPS were obtained and their antitumor activities were compared by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results showed that when DS within the scope of 0.81-1.29, carbohydrate content in the range of 41.41-78.56%, sulfated derivatives exhibit relatively strong antitumor activity in vitro. The optimum modification conditions were reaction temperature of 70 degrees C, the ratio of chlorosulfonic acid to pyridine of 1:4 and the reaction time of 2h.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antineoplastic Agents / pharmacology*
Cell Line, Tumor
Cell Proliferation / drug effects
Drug Screening Assays, Antitumor
Fatty Acids / isolation & purification*
Humans
Mice
Oryza / chemistry*
Polysaccharides / chemistry
Polysaccharides / pharmacology*
Sulfates / metabolism*

Substances

Antineoplastic Agents
Fatty Acids
Polysaccharides
Sulfates