From October 2005 through September 2006, blood samples collected from 1,412 (768 male, 644 female) 1-yr-old and older stray dogs in Taipei City, Taiwan, were tested for antibodies to Toxoplasma gondii using the latex agglutination test (LAT). Antibodies (LAT titers >1:32) to T. gondii were found in 284 (20.1%) of the animals. Seroprevalences were not affected by the sex or density of dogs. The prevalence was highest in dogs from the eastern part of the city.