Chemical imaging with Fourier transform coherent anti-Stokes Raman scattering microscopy

Appl Opt. 2008 Nov 1;47(31):5790-8. doi: 10.1364/ao.47.005790.

Abstract

We report chemical imaging using Fourier transform coherent anti-Stokes Raman scattering (FTCARS) microscopy. Adding a passively phase-stable local field to amplify the weak FTCARS signal, we also demonstrate interferometric FTCARS microscopy, permitting reduced incident power to be used for imaging. We discuss signal-to-noise considerations and the conditions necessary to effectively suppress background noise, allowing FTCARS microscopy that is limited by the shot noise of the detector. We also discuss differences between the signal-to-noise obtainable by time and frequency domain coherent anti-Stokes Raman scattering (CARS) methods.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Equipment Design
  • Interferometry / methods
  • Microscopy / methods
  • Models, Statistical
  • Optics and Photonics
  • Polystyrenes / chemistry
  • Scattering, Radiation
  • Spectroscopy, Fourier Transform Infrared / methods*
  • Spectrum Analysis, Raman / methods*
  • Time Factors
  • Water / chemistry

Substances

  • Polystyrenes
  • Water