1 Cell Biology and Metabolism Program, National Institute of Child Health and Human Development, National Institutes of Health, 18 Library Drive, Bethesda, MD 20892, USA. lippincj@mail.nih.gov
Super-resolution microscopy is poised to revolutionize our understanding of the workings of the cell. But the technology still has some limitations, and these must be taken into consideration if widespread application is to yield biological insight.