Vital dye dil and fluorescent magnetic microparticles do not affect the phenotype of mesenchymal stem cells from human amnion and their differentiation capacity

I B Cheglakov; A N Rytenkov; K N Yarygin

doi:10.1007/s10517-008-0129-z

Vital dye dil and fluorescent magnetic microparticles do not affect the phenotype of mesenchymal stem cells from human amnion and their differentiation capacity

Bull Exp Biol Med. 2008 Apr;145(4):504-10. doi: 10.1007/s10517-008-0129-z.

Authors

I B Cheglakov¹, A N Rytenkov, K N Yarygin

Affiliation

¹ V. N. Orekhovich Research Institute of Biomedical Chemistry, Russia. cheglakovib@yahoo.ca

PMID: 19110605
DOI: 10.1007/s10517-008-0129-z

Abstract

We present a method of labeling of mesenchymal stem cells from human amnion with a fluorescent dye Dil and microspheres (Bangs Laboratories). The possibility of administration of loaded cell culture was verified and comparative analysis of the phenotype of mesenchymal stem cells by the expression of fibronectin, nestin, CD13, CD29, CD34, CD44, CD54, CD90, CD105, CD106, HLA-ABC, HLA-DR, and PCNA was carried out. The labeled cells retained osteogenic differentiation capacity. The results suggest that fluorescent dye Dil and microspheres from Bangs Laboratories can be used for monitoring of mesenchymal stem cells from human amnion in in vivo experiments.

Publication types

Evaluation Study

MeSH terms

Amnion / cytology*
Cell Differentiation / drug effects*
Cell Proliferation / drug effects
Cell Survival / drug effects
Cells, Cultured
Fluorescent Dyes / pharmacology*
Humans
Magnetics
Mesenchymal Stem Cells / cytology
Mesenchymal Stem Cells / drug effects*
Mesenchymal Stem Cells / physiology
Microspheres*
Osteogenesis / drug effects
Particle Size
Phenotype
Staining and Labeling / methods

Substances

Fluorescent Dyes