Abstract
CD160 has been initially identified as a GPI-anchored MHC-class I activating receptor mainly expressed on peripheral blood NK cells. Herein, we report the identification of three additional CD160-related mRNAs generated through alternative splicings of the CD160 gene, among which one encoded a putative CD160 transmembrane isoform (CD160-TM). We first establish that CD160-TM surface expression is highly restricted to NK cells and is activation-dependent. Additionally, we provide evidence that CD160-TM represents a novel activating receptor, as assessed by the increased CD107a NK cell surface mobilization observed upon its engagement. Finally, we demonstrate that the CD160-TM cytoplasmic tail is by itself sufficient to mediate the recruitment of Erk1/2 signaling pathway, and that the initiation of this activation process is dependent on the Src-family kinase p56(lck). The identification of CD160-TM therefore provides new possibilities regarding the role of CD160 isoforms in the regulation of NK cell functions.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Antigens, CD / biosynthesis
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Antigens, CD / genetics
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Antigens, CD / isolation & purification*
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Antigens, CD / physiology
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Base Sequence
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CD8-Positive T-Lymphocytes / immunology
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Cell Line
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Cell Line, Tumor
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Clone Cells
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GPI-Linked Proteins
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Gene Expression Regulation / immunology*
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Humans
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Jurkat Cells
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Killer Cells, Natural / cytology
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Killer Cells, Natural / immunology*
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Killer Cells, Natural / metabolism*
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Lymphocyte Activation / genetics
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Lymphocyte Activation / immunology*
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Membrane Proteins / biosynthesis
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Membrane Proteins / genetics
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Membrane Proteins / isolation & purification*
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Membrane Proteins / physiology*
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Molecular Sequence Data
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Protein Isoforms / biosynthesis
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Protein Isoforms / genetics
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Protein Isoforms / isolation & purification
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Protein Isoforms / physiology
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RNA, Messenger / isolation & purification
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Receptors, Immunologic / biosynthesis
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Receptors, Immunologic / genetics
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Receptors, Immunologic / isolation & purification*
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Receptors, Immunologic / physiology
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Resting Phase, Cell Cycle / genetics
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Resting Phase, Cell Cycle / immunology
Substances
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Antigens, CD
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CD160 protein, human
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GPI-Linked Proteins
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Membrane Proteins
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Protein Isoforms
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RNA, Messenger
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Receptors, Immunologic
Associated data
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GENBANK/EU016100
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GENBANK/EU016101