Antibodies raised against individual viral envelope proteins have been used in white spot syndrome virus (WSSV) neutralization assays. We report here the sequence analysis of cDNAs encoding the variable regions of a novel monoclonal antibody that binds to the viral envelope protein and neutralizes WSSV infection. The heavy and light variable chains are most homologous to VH7183 germline gene (AF120472) and IgVk RF germline gene (AJ235936), respectively. Database searches using the derived sequences predicted residues comprising CDR loops. The 12 amino acid residue of the heavy chain CDR3 is rich in negatively charged aspartic acid (25%) and did not show significant homology to any murine V gene available on the database. This study provides insights on the paratope-epitope interaction and can be used to identify compounds with comparable properties as the paratope leading to future development of drugs and vaccines for WSSV infection.