Neurogenesis induced by seizures in the dentate gyrus is not related to mossy fiber sprouting but is age dependent in developing rats

Arq Neuropsiquiatr. 2008 Dec;66(4):853-60. doi: 10.1590/s0004-282x2008000600015.

Abstract

Neurogenesis in the dentate gyrus (DG) has attracted attention since abnormal supragranular mossy fiber sprouting occurs in the same region, in temporal lobe epilepsy. Thus, we submitted developing rats to pilocarpine-induced status epilepticus (SE) to study the relationship between neurogenesis and mossy fiber sprouting. Groups were submitted to SE at: I-P9, II-P7, P8 and P9, III-P17 e IV-P21. Neurogenesis was quantified using BrdU protocol and confirmed through double staining, using neuronal pentraxin. Other animals were monitored by video system until P120 and their brain was studied (Timm and Nissl staining). The neurogenesis at P17 (p=0.007) and P21 (p=0.006) were increased. However, only P21 group showed recurrent seizures and the mossy fiber sprouting in the same region, during adult life, while P17 did not. Thus, our results suggest that neurogenesis is not related to mossy fiber sprouting neither to recurrent spontaneous seizures in pilocarpine model.

MeSH terms

  • Animals
  • Cell Proliferation / drug effects
  • Dentate Gyrus / drug effects
  • Dentate Gyrus / embryology
  • Dentate Gyrus / physiopathology*
  • Immunohistochemistry
  • Mossy Fibers, Hippocampal / drug effects
  • Mossy Fibers, Hippocampal / embryology
  • Mossy Fibers, Hippocampal / physiopathology
  • Neurogenesis / physiology*
  • Neuronal Plasticity
  • Pilocarpine
  • Rats
  • Rats, Sprague-Dawley
  • Status Epilepticus / chemically induced
  • Status Epilepticus / physiopathology*

Substances

  • Pilocarpine