Abstract
A quinoline sensitizer-centered lanthanide chelate system of novel design for TR-LRET was prepared; it exhibited high labelling efficiency with a his-tagged protein (ERalpha-LBD) on the Ni-NTA beads, using a mixed metal chelate protocol, and it functioned well in TR-LRET protein-protein interaction assays.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Chelating Agents / chemistry*
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Energy Transfer*
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Fluorescence Resonance Energy Transfer
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Lanthanoid Series Elements / chemical synthesis
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Lanthanoid Series Elements / chemistry*
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Microspheres
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Models, Chemical
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Nitrilotriacetic Acid / analogs & derivatives*
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Nitrilotriacetic Acid / chemistry
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Organometallic Compounds / chemistry*
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Proteins / chemistry*
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Quinolines / chemical synthesis
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Quinolines / chemistry*
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Staining and Labeling / methods*
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Time Factors
Substances
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Chelating Agents
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Lanthanoid Series Elements
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Organometallic Compounds
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Proteins
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Quinolines
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nickel nitrilotriacetic acid
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Nitrilotriacetic Acid