The present paper reviews recent progress about the application of rat's praxiology evaluation in studies on the mechanism of acupuncture in the treatment of some diseases from 1) motor functional impairment evaluation; 2) cognition impairment evaluation; 3) animal mood score, and 4) pain behavior evaluation. By using different praxiology evaluation methods, the acupuncture curative effect has been confirmed repeatedly in different pathological models. The animal experimental study plays an important role in understanding the mechanisms of acupuncture in the treatment of some common diseases in clinic. However, due to the multiplicity of diseases, and specificity of various phases of a disease, extensive and specific behavior evaluation methods are relatively fewer, some current method terms are still obscure and manual of operations is deficient. Therefore, further improvement of the current behavior evaluation methods and developing more methods with stronger and specific functions aiming at different experiments (disease models) are definitely necessary.