Objective: To investigate the relationship between the expression of neuronal acetylcholine receptor alpha 7 (nAChRalpha7) and the role of nAChRalpha7 in the development of asthma by detecting the expression of nAChR alpha7 on CD(4)(+) T lymphocytes in the peripheral blood and the level of cytokines IFN-gamma and IL-4 in the blood serum from children with asthma.
Methods: Thirty patients in the asthma group and 20 healthy children in the normal control group were included. Peripheral blood was collected, and the serum was harvested. The expression of nAChRalpha7 on CD(3)(+) and CD(4)(+) cells were detected by flow cytometry. The levels of IFN-gamma and IL-4 in the serum were detected by ELISA.
Results: The expression of nAChRalpha7 on CD(4)(+) T lymphocytes from children with asthma was higher than that from normal controls (t = 2.53, P < 0.05). Compared with the control group, the level of IL-4 in the serum of the asthma group was higher (t = 5.42, P < 0.01) but the level of IFN-gamma was lower (t = 4.11, P < 0.01). There were positive correlation between nAChRalpha7 and IL-4 (r = 0.688, P < 0.01) and negative correlation between nAChRalpha7 and IFN-gamma (r = -0.476, P < 0.01).
Conclusion: nAChRalpha7 was shown to influence the balance of Th1/Th2 and it may play an important role in the development of asthma.