Simultaneous determination of sorbic and benzoic acids in commercial juices using the PLS-2 multivariate calibration method and validation by high performance liquid chromatography

Valeria A Lozano; José M Camiña; María S Boeris; Eduardo J Marchevsky

doi:10.1016/j.talanta.2007.03.041

Simultaneous determination of sorbic and benzoic acids in commercial juices using the PLS-2 multivariate calibration method and validation by high performance liquid chromatography

Talanta. 2007 Sep 15;73(2):282-6. doi: 10.1016/j.talanta.2007.03.041. Epub 2007 Mar 25.

Authors

Valeria A Lozano¹, José M Camiña, María S Boeris, Eduardo J Marchevsky

Affiliation

¹ Departamento de Química, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de La Pampa, Av. Uruguay 151, 6300 Santa Rosa, La Pampa, Argentina.

PMID: 19073029
DOI: 10.1016/j.talanta.2007.03.041

Abstract

A new method to determine a mixture for preserving sorbic and benzoic acids in commercial juices is proposed. The PLS-2 model was obtained preparing 40 standard solutions adding concentration of sorbic and benzoic acid to filtered natural juices of apple, lemon, orange and grapefruit. The concentration of analytes in the commercial samples was evaluated using the obtained model by UV spectral data. The PLS-2 method was validated by high performance liquid chromatography (HPLC), finding a relative error less than 12% between the PLS-2 and HPLC methods in all cases.