Combination of different molecular mechanisms leading to fluconazole resistance in a Candida lusitaniae clinical isolate

Diagn Microbiol Infect Dis. 2009 Feb;63(2):188-93. doi: 10.1016/j.diagmicrobio.2008.10.019. Epub 2008 Dec 12.

Abstract

We report on the underlying molecular mechanisms likely responsible for the high-level fluconazole resistance in a Candida lusitaniae clinical isolate. Fluconazole resistance correlated with overexpression of ERG11 and of several efflux pump genes, in particular, the orthologs of the Candida albicans MDR1, PDR16, CDR1, CDR2, and YOR1.

MeSH terms

  • ATP-Binding Cassette Transporters / genetics
  • Candida / drug effects*
  • Candida / genetics*
  • Cytochrome P-450 Enzyme System / genetics
  • DNA Primers
  • Drug Resistance, Fungal / genetics*
  • Fluconazole / pharmacology*
  • Fungal Proteins / genetics
  • Gene Expression Regulation, Fungal / drug effects*
  • Humans
  • Membrane Transport Proteins / genetics
  • Methyltransferases / genetics
  • Oxidoreductases / genetics
  • Polymerase Chain Reaction / methods

Substances

  • ATP-Binding Cassette Transporters
  • DNA Primers
  • Fungal Proteins
  • Membrane Transport Proteins
  • Fluconazole
  • Cytochrome P-450 Enzyme System
  • Oxidoreductases
  • Methyltransferases