It has been well established that mutations of the solute carrier family 11 A1 (SLC11A1) gene are responsible for susceptibility to a number of intracellular pathogens. Mastitis is the most common and most costly disease of dairy cattle, commonly caused by bacteria. As SLC11A1 protein is involved in bacterial killing, we assumed SLC11A1 gene as a candidate gene for bovine mastitis resistance. In this study, polymorphisms in the SLC11A1 gene were identified in the Chinese Holstein population. Genotypes were constructed, and their associations with somatic cell score and milk yield were determined. Single-strand conformation polymorphisms and DNA sequencing were used to reveal polymorphisms in SLC11A1 gene. A SNP in coding region and a variation of GT repeats in 3'-untranslated region were detected. The SNP resulted in an amino acid variation of p.P356A in transmembrane 8 of SLC11A1 peptide, which is the most conserved consecutive region. However, no associations were observed between the detected polymorphisms and somatic cell scores and milk yield in the Chinese Holstein population.