Objective: To study the effect of hydroquinone (HQ) on expression of XPV mRNA in L-02 hepatic cells and its regularity, in order to explore the possible molecular mechanism of XPV in the process of the toxic effects of HQ to L-02 hepatic cells.
Methods: Expression of XPV mRNA in L-02 hepatic cells treated at the dose of 40 micromol/L HQ for 6, 12, 24 and 48h in vitro and its expression in L-02 hepatic cells treated at the concentrations of 0, 5, 10, 20, 40, 80 and 160 micromol/L HQ for 24h was measured by real-time fluorescence quantitative PCR.
Results: In the range of 0-24h, the expressions of XPV mRNA in L-02 hepatic cells in treated group (40 micromol/L) and control group increased with increase of time, and expressions of XPV reached the maximum levels at the times of 24h,and reduced the minimum levels at the times of 48h. At the time ranges of 6, 12, 24 and 48h,the expression levels of XPV mRNA in L-02 hepatic cells treated with HQ (40 micromol/L) were more higher than those of the control (0 micromol/L). The expression level of XPV mRNA in L-02 hepatic cells treated by HQ at the concentration of 0-80 micromol/L for 24h increased in a dose-dependent manner.Taken the expression level of the control group as 1, the relative expression levels in various treatment groups were added to 1.20, 2.02, 2.37, 2.67, 4.40 and 2.32 fold respectively. The expressions of XPV mRNA in the group treated with HQ (160 micromol/L) was reduced, but was higher than that of the control.
Conclusion: HQ could induce the expression of XPV at mRNA levels in a dose- and time-dependent manner, which indicated that XPV could involved in the response of L-02 hepatic cells of HQ.