Accumulation of the bioactive peptides, novokinin, LPYPR and rubiscolin, in seeds of genetically modified soybean

Biosci Biotechnol Biochem. 2008 Dec;72(12):3301-5. doi: 10.1271/bbb.80468. Epub 2008 Dec 7.

Abstract

Novokinin (RPLKPW), LPYPR, and rubiscolin (YPLDLF) are bioactive peptides with respective hypotensive, hypocholesterolemic, and memory-enhancing activities. We generated transgenic soybean lines that expressed modified forms of the alpha' subunit of seed storage protein beta-conglycinin containing tandem repeats of these bioactive peptides. The modified alpha' subunits constituted up to 0.2% of extracted proteins from the transgenic seeds.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Food, Genetically Modified
  • Glycine max / genetics*
  • Glycine max / metabolism*
  • Mice
  • Molecular Sequence Data
  • Oligopeptides / chemistry
  • Oligopeptides / metabolism*
  • Seeds / metabolism*

Substances

  • Arg-Pro-Leu-Lys-Pro-Trp
  • Oligopeptides