Taxonomic study of a chromomycin-producing strain and reclassification of Streptomyces cavourensis subsp. washingtonensis as a later synonym of Streptomyces griseus

Int J Syst Evol Microbiol. 2008 Dec;58(Pt 12):2783-7. doi: 10.1099/ijs.0.65628-0.

Abstract

A chromomycin-producing actinomycete, strain AP19, was isolated from a sample of faeces collected from Foping national nature reserve in China. Chemotaxonomic and morphological properties indicated that the novel isolate was a member of the genus Streptomyces. Phylogenetic analyses based on an almost complete 16S rRNA gene sequence of the strain and on the 120-nt nucleotide variable gamma-region of this molecule revealed that it was closely related to Streptomyces griseus ISP 5236(T) and Streptomyces cavourensis subsp. washingtonensis ATCC 27732(T). DNA-DNA relatedness values among these strains were above 70 %. Streptomyces cavourensis subsp. washingtonensis could be readily distinguished from Streptomyces cavourensis ATCC 14889(T) by differing BOX-PCR fingerprinting patterns, relatively low 16S rRNA gene sequence similarity and a low DNA-DNA relatedness value. It is proposed, therefore, that Streptomyces cavourensis subsp. washingtonensis is a later synonym of Streptomyces griseus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromomycins / metabolism*
  • Classification
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Species Specificity
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Streptomyces / chemistry
  • Streptomyces / classification*
  • Streptomyces / genetics
  • Streptomyces / physiology*
  • Streptomyces griseus / chemistry
  • Streptomyces griseus / classification*
  • Streptomyces griseus / genetics
  • Streptomyces griseus / physiology*

Substances

  • Chromomycins
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/EF466100