Surface-enhanced Raman scattering imaging of HER2 cancer markers overexpressed in single MCF7 cells using antibody conjugated hollow gold nanospheres

Sangyeop Lee; Hyangah Chon; Moonkwon Lee; Jaebum Choo; Soon Young Shin; Young Han Lee; Im Joo Rhyu; Sang Wook Son; Chil Hwan Oh

doi:10.1016/j.bios.2008.10.018

Surface-enhanced Raman scattering imaging of HER2 cancer markers overexpressed in single MCF7 cells using antibody conjugated hollow gold nanospheres

Biosens Bioelectron. 2009 Mar 15;24(7):2260-3. doi: 10.1016/j.bios.2008.10.018. Epub 2008 Nov 5.

Authors

Sangyeop Lee¹, Hyangah Chon, Moonkwon Lee, Jaebum Choo, Soon Young Shin, Young Han Lee, Im Joo Rhyu, Sang Wook Son, Chil Hwan Oh

Affiliation

¹ Department of Applied Chemistry, Hanyang University, Ansan 426-791, South Korea.

PMID: 19056254
DOI: 10.1016/j.bios.2008.10.018

Abstract

Antibody-conjugated hollow gold nanospheres (HGNs) have been used for the SERS imaging of HER2 cancer markers overexpressed in single MCF7 cells. SERS mapping images show that HGNs have much better homogeneous scattering properties than silver nanoparticles. The results demonstrate the potential feasibility of HGNs as highly sensitive and homogeneous sensing probes for biological imaging of cancer markers in live cells.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Antibodies*
Biomarkers, Tumor / immunology
Biomarkers, Tumor / metabolism
Breast Neoplasms / immunology
Breast Neoplasms / metabolism*
Breast Neoplasms / pathology*
Cell Line, Tumor
Contrast Media / chemistry
Gold* / chemistry
Humans
Image Enhancement / methods
Microscopy / methods
Nanostructures* / chemistry
Nanostructures* / ultrastructure
Receptor, ErbB-2 / metabolism*
Spectrum Analysis, Raman / methods*

Substances

Antibodies
Biomarkers, Tumor
Contrast Media
Gold
ERBB2 protein, human
Receptor, ErbB-2