It has been shown that Pancreatic Stem Cells (PSCs) share many features with skin stem cells. Yet, their potential role in skin regeneration remains to be elucidated. 5x10(5) PSCs from male Rattus norwegicus were seeded on Matriderm scaffold overnight. Cells survival and proliferation were then tested in vitro showing the survival of the cells and their homogenous distribution in the scaffolds. Afterwards, scaffolds were used to replace bilateral full-thickness skin wounds made on the dorsum of Nu/Nu mice. A control group of nude mice received the Matriderm scaffolds without cells. Two weeks after transplantation, wound areas were harvested and analyzed with respect to epithelialization, vascularization and wound closure. The healing area and regeneration rate were significantly increased in the group used the PSCs-seeded scaffolds (factor of 2.1). Vascularization rate showed a significant increase in the PSCs-seeded scaffolds(factor of 1.5). Morphology and immunohistochemistry showed new skin-like structures positive to epidermal markers in the healing wound bed. PSCs were detected in the regenerated tissues. This study showed that the combined use of PSCs with the Matriderm as a scaffold for dermal regeneration significantly increased the epidermalization, vascularization and healing in full-thickness wounds.