Surface engineering of poly(D,L-lactic acid) by entrapment of soluble eggshell membrane protein

J Biomed Mater Res A. 2009 Dec;91(3):701-7. doi: 10.1002/jbm.a.32304.

Abstract

Soluble eggshell membrane protein (SEP) is used to engineer poly(D,L-lactic acid) (PDL-LA) membrane surface by a physical entrapment method to enhance cytocompatibility. Attenuated total reflection Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and contact angle measurements are used to characterize SEP-modified PDL-LA membrane surface and confirm the formation of an even and stable layer of SEP on PDL-LA membrane surface. NIH3T3 cell lines are used as model cells to evaluate cytocompatibility of the SEP-modified PDL-LA membrane, which is much better than that of virgin PDL-LA membrane. The enhanced cytocompatibility facilitates potential application in tissue engineering.

MeSH terms

  • Animals
  • Biocompatible Materials / chemistry*
  • Cell Adhesion
  • Cross-Linking Reagents / chemistry
  • Egg Shell
  • Glutaral / chemistry
  • Lactic Acid / chemistry*
  • Materials Testing
  • Mice
  • NIH 3T3 Cells
  • Polyesters
  • Polymers / chemistry*
  • Protein Engineering / methods*
  • Spectroscopy, Fourier Transform Infrared / methods
  • Surface Properties
  • Temperature
  • Tissue Engineering / methods

Substances

  • Biocompatible Materials
  • Cross-Linking Reagents
  • Polyesters
  • Polymers
  • Lactic Acid
  • poly(lactide)
  • Glutaral