Identification of 2-[2-nitro-4-(trifluoromethyl)benzoyl]- cyclohexane-1,3-dione metabolites in urine of patients suffering from tyrosinemia type I with the use of 1H and 19F NMR spectroscopy

Przemysław Szczeciński; Diana Lamparska; Adam Gryff-Keller; Wanda Gradowska

Identification of 2-[2-nitro-4-(trifluoromethyl)benzoyl]- cyclohexane-1,3-dione metabolites in urine of patients suffering from tyrosinemia type I with the use of 1H and 19F NMR spectroscopy

Acta Biochim Pol. 2008;55(4):749-52. Epub 2008 Nov 28.

Authors

Przemysław Szczeciński¹, Diana Lamparska, Adam Gryff-Keller, Wanda Gradowska

Affiliation

¹ Faculty of Chemistry, Warsaw University of Technology, Warszawa, Poland. przemsz@ch.pw.edu.pl

PMID: 19039335

Abstract

Organic extracts of six urine samples from children treated with nitisinone, a medicine against tyrosinemia type I, were investigated by (1)H and (19)F NMR spectroscopy. The presence of unchanged 2-[2-nitro-4-(trifluoromethyl)benzoyl]cyclohexane-1,3-dione (NTBC), 6-hydroxy-2-[2-nitro-4-(trifluoromethyl)benzoyl]cyclohexane-1,3-dione (NTBC-OH) and 2-nitro-4-trifluoromethylbenzoic acid (NTFA) as well as a few other unidentified compounds containing CF(3) group was documented.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Child
Child, Preschool
Cyclohexanones / chemistry*
Female
Fluorine
Humans
Magnetic Resonance Spectroscopy / methods*
Male
Nitrobenzoates / chemistry*
Protons
Tyrosinemias / urine*

Substances

Cyclohexanones
Nitrobenzoates
Protons
Fluorine
nitisinone