Assessment of a microplate method for detection of staphylococcal secretory inhibitor of platelet microbicidal protein

Iuri B Ivanov; Viktor A Gritsenko

doi:10.1016/j.diagmicrobio.2008.09.011

Assessment of a microplate method for detection of staphylococcal secretory inhibitor of platelet microbicidal protein

Diagn Microbiol Infect Dis. 2009 Jan;63(1):118-20. doi: 10.1016/j.diagmicrobio.2008.09.011. Epub 2008 Nov 21.

Authors

Iuri B Ivanov¹, Viktor A Gritsenko

Affiliation

¹ Department of Human Microbiology, Institute of Cellular and Intracellular Symbiosis, Russian Academy of Sciences, Orenburg 460056, Russia. mil-for@rambler.ru

PMID: 19026508
DOI: 10.1016/j.diagmicrobio.2008.09.011

Abstract

We developed a novel microplate spectrophotometric assay for the detection of staphylococcal secretory inhibitor of platelet microbicidal protein (SIPMP) in <or=24 h. The levels of SIPMP production agreed with the results obtained by viable counting method. Both methods may be acceptable and cheap, allowing for their routine use in clinical laboratories.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / pharmacology*
Bacteriological Techniques / methods*
Blood Bactericidal Activity / drug effects*
Blood Platelets / metabolism
Chronic Disease
Humans
Male
Prostate / microbiology
Prostatitis / microbiology*
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry / methods
Staphylococcal Infections / microbiology
Staphylococcus / metabolism*
beta-Thromboglobulin / antagonists & inhibitors*

Substances

Bacterial Proteins
PPBP protein, human
beta-Thromboglobulin